Rapid isolation of genomic DNA from gram-negative bacteria.

Comparison of three DNA extraction methods for polymerase chain reaction (PCR) analysis of bacterial genomic DNA

Rapid isolation of high-purity microbial genomic DNA is necessary for genome analysis. In this study, the authors compared a one-hour procedure using a microwave with enzymatic and boiling methods of genomic DNA extraction from Gram-negative and Gram-positive bacteria. High DNA concentration and purity were observed for both MRSA and ESBL strains (80.1 and 91.1 μg/ml; OD260/280, 1.82 and 1.70, ...

Isolation of Genomic Dna from Gram Negative Bacteria

Genomic flank-sequencing of plasposon insertion sites for rapid identification of functional genes.

Plasposons are modified mini-Tn5 transposons for random mutagenesis of Gram-negative bacteria. Their unique design allows for the rescue cloning and sequencing of DNA that flanks insertion sites in plasposon mutants. However, this process can be laborious and time-consuming, as it involves genomic DNA isolation, restriction endonuclease treatment, subsequent religation, transformation of religa...

Simultaneous Detection of Helicobacter Genus and Helicobacter pylori Species using a Multiplex PCR Method

In order to improve simultaneous detection and identification of Helicobacter genus in general and Helicobacter pylori specifically and reduce the number of amplifications needed, we established a multiplex PCR. In this study, two pairs of primers: Hcom1 and Hcom2 specific for Helicobacter genus, Hicd1 and Hicd2 specific for Helicobacter pylori species were used. To determine the sensitivity of...

Isolation of ASR7 Actinomycete Isolated from S12 Demospongia Marine Sponge and Study of Its Antibacterial Activity

Background and Aims: Marine Actinomycetes are gram-positive bacteria that sometimes are free, saprophytic or plant and animal-associated, including marine sponges. More than 75% of antibiotics and antimicrobial compounds are produced by actinomycetes. In recent years, due to the need for new drugs, marine microorganisms have been considered as new sources of potential production of significant ...